Cilia structure and intraflagellar transport differentially regulate sensory response dynamics within and between C. elegans chemosensory neurons

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Alison Philbrook;Michael P. O'Donnell;Laura Grunenkovaite;Piali Sengupta

Description

List of data with corresponding filesFigure 1A)    File name: Figure 1A- ASH_IFT_mutants_length_copy.pzfxB)    File names: (Folder) ASH_1Mglycerol_IFT_mutants and (Folder) ASH_10-2IAA_IFT_mutantsC)    File names: (Folder) ASH_1Mglycerol_IFT_mutants > Peak_responses and (Folder) ASH_10-2IAA_IFT_mutants > Peak_responses - first pulseD)   File names: Figure 1D – ASH Cher and Marianas Lengths Truncation osm-3(ts) 27 and 30 degree graph and Figure 1D – ASH Cher and Marianas Lengths Truncation osm-3(ts) 27 degreesE)    File name: Figure 1E – ASH IFT Quantification.xlsxF)    File name: (Folder) ASH_1Mglycerol_osm3tsG)   File names: Figure 1 – Glycerol_Assays_Data.pzfx and Figure 1 – Glycerol_Assays_Data.xlsxH)   File name: Figure 1H – ALL ASH Marianas and Cher Lengths che-3(nx159ts)I)     File names: Figure 1- ASH IFT PY12007 v AP457 and Figure 1- ASH IFT PY12007 v AP457.xlsxJ)    File name: (Folder) ASH_1Mglycerol_che3tsK)   File names: Figure 1 – Glycerol_Assays_Data.pzfx and Figure 1 – Glycerol_Assays_Data.xlsxFigure 2B)  File name: (Folder) 10-7Diacetyl_Calcium_IFT_mutantsC)  File names: (Folder) 10-6Pyrazine_Calcium_IFT_mutants and (Folder) 10-7Pyrazine_Calcium_IFT_mutantsD)  File names: (Folder) Microfluidics > (Folder) 10-6pyrazine and (Folder) 10-7diacetylE)  File names: (Folder) Microfluidics > Diacetyl_Chemotaxis_index_osm6_osm3kap1.pzfx and Pyrazine_Chemotaxis_index_pyrazine_osm3kap1_osm6.pzfxG)  File name: (Folder) 10-6Pyrazine_Calcium_misexpressionFigure 3A)   File name: Figure 3A- AWA IFT AP303 v AP379.xlsxC)  File names: (Folder) AWA_Calcium_Diacetyl and (Folder) AWA_Calcium_PyrazineD)  File names: (Folder) AWA_Calcium_Diacetyl and (Folder) AWA_Calcium_PyrazineE)  File name: Figure 3E- SRX64 and ODR10 within dendritic branches.pzfxFigure 4A)   File name: (Folder) AWA_Calcium_Diacetyl_grk2B)   File name: (Folder) AWA_Calcium_Pyrazine_grk2C)   File name: (Folder) Microfluidics_diacetylD)   File name: (Folder) Microfluidics_pyrazineE)    File name: Figure 4E- GRK2tRFP_kap1osm3ts.pzfxFigure 5A)   File names: (Folder) AWA_Diacetyl_Adaptation_osm3ts and (Folder) AWA_Pyrazine_Adaptation_osm3tsB)    File name: Figure 5B and S7B- FRAP.pzfxC)    File names: Figure 5C- SRX64_bbs7jhu590.pzfx and Figure 5C- SRX64_bbs7ok1351.pzfx and Figure 5C- oy158_bbs7jhu590.pzfx and Figure 5C- oy158_bbs7ok1351.pzfxD)   File names: (Folder) AWA_Diacetyl_Adaptation_bbs7 and (Folder) AWA_Pyrazine_Adaptation_bbs7E)    File name: Figure 5E- Marianas_EV_ODR10_SRX64_osm3tskap1.pzfxFigure S1A)   File names: (Folder) ASH_IFTmutants_0.5and2Mglycerol > (Folder) 0.5Mglycerol and (Folder) 2MglycerolB)    File names: (Folder) ASH_IFTmutants_quinine > (Folder) 5mM_quinine and (Folder) 10mM_quinineC)    File name: ASH_IAA_t1/2D)   File names: (Folder) ASH_IFTmutants_heptanol > (Folder) 10-3_heptanol and (Folder) 10-4_heptanolE)    File names: (Folder) ASH_IFTmutants_2Mglycerol_GABA and (Folder) ASH_IFTmutants_2Mglycerol_bicucullineFigure S2B)    File name: Figure S2B- Dye-filling_dataC)    File name: Figure S2C- ASH_IFT_mutants_length.pzfxD)   File names: Figure S2D- ASH IFT PY6345 v PY12007 and Figure S2D- ASH IFT QuantificationFigure S3A)   File name: (Folder) AWA_10-7Diacetyl_IFTmutantsB)    File names: (Folder) AWA_Calcium_heptanone > (Folder) 10-4_heptanone and (Folder) 10-5_heptanoneC)    File name: (Folder) AWA_10-5Pyrazine_IFTmutantsD)   File names: (Folder) AWA_Calcium_methylpyrazine > (Folder) 10-8_methylpyrazine and (Folder) 10-9_methylpyrazineE)    File name: Figure S3- Chemotaxis IFT_mutants.pzfxFigure S4A)  File name: Figure S4A – AP419_pyrazine_Chemotaxis.pzfxC)  File name: Figure S4C – srx64SL2gfp_osm5.pzfxFigure S6A)   File names: Diacetyl_prism_decay_grk2 and Pyrazine_prism_decay_grk2Figure S7 A)  File name: (Folder) AWA_Calcium_Adaptation_diacetyl_IFT_mutant B)  File name: Figure 5B and S7B- FRAP C)  File name: Figure S7C- oy158_grk2_rescue.pzfx F)  File names: (Folder) AWA_Calcium_Adaptation_diacetyl_grk2 > (Folder) grk2 and (Folder) grk2bbs7doubleAdditional materials            All code is listed under Code from Github > MF.matR-master and Additional Methods

Citations (1)

Mentions (0)

Metrics

Dataset Index

2.0

FAIR Score

69%

Citations

1

Mentions

0

Metrics Over Time

Publication Details

DOI

Publisher

Zenodo

Assigned Domain

Subfield

Clinical Biochemistry

Field

Biochemistry, Genetics and Molecular Biology

Domain

Life Sciences

Confidence Score

32%

Source

Scholar Data Model

Normalization Factors

FT

13.46

CTw

1.00

MTw

1.00