Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent supplementary material description.

Estrogen receptor alpha (ESR1) gene amplification is frequent in breast cancer. Subsequent supplementary material. Frederik Holst & Christian Ruiz The supplementary material refers to: Holst, F., Stahl, P.R., Ruiz, C., et al. Estrogen receptor alpha (ESR1) gene amplification s frequent in breast cancer. Nature Genetics 39, 655-660 (2007). Subsequent Supplementary Figures S1-S7 Illustration of the different Affymetrix 10K SNP array gene copy number data analysis methods and regarding segmented ESR1 copy number profiles of 22 breast cancers. Taken from Subsequent Supplementary Optical Dataset S1 a photographical documentation of ESR1 FISH analysis signal appearance of tumors with ESR1 amplification by SNP GeneChip analysis is added (figures 5A-C + 7A-C). DOI: http://dx.doi.org/10.6084/m9.figshare.1470035 Subsequent Supplementary Video Clips S1+S2 The video clips document FISH analysis as performed for results documented in regarding subsequent supplementary figures 5A-C + 7A-C. Video clips are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 x 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1471677 Subsequent Supplementary Optical Dataset S1 The optical dataset consists of 94 photographs that show the appearance of ESR1 FISH signals of 20 out of 22 tumors in regard to the GeneChip analysis that is illustrated in subsequent supplementary figures S1-S3 and S4+S6. The 94 photographs are separated into 1-10 figures per tumor including up to three z-stacks per figure. Photographs are taken using an Olympus IX51 microscope at 100x magnification, an Olympus XM10 digital camera (1,376 ´ 1,032 pixels) and Olympus cellSens imaging software. DOI: http://dx.doi.org/10.6084/m9.figshare.1470130 Methods References Ruiz, C. Doctoral Thesis, Universität Basel (2006). Moelans, C.B., Holst, F., Hellwinkel, O., Simon, R. & van Diest, P.J. ESR1 Amplification in Breast Cancer by Optimized RNase FISH: Frequent but Low-Level and Heterogeneous. PLoS One 8, e84189 (2013). Holst, F. FISH Analysis of ESR1 Amplification in Breast Cancer - A Video Illustration. DOI: http://dx.doi.org/10.6084/m9.figshare.1471765 (2015).