Additional file 1: Figure S1. of Micro-anatomical quantitative optical imaging: toward automated assessment of breast tissues

Illustration of methodology used to select MSER intensity parameters. The intensity parameters MaxVariation, MinDiversity, and Delta were selected by varying each parameter one at a time. The area fraction (AF) was calculated for each representative image after each iteration of MSER. Each intensity parameter was plotted as a function of AF. Values for the intensity parameters were selected based on which values correctly isolated nuclei from the representative images and which values led to the largest differences between tumor and benign images. Scale bar 100Â Îźm. (ZIP 140 kb)