Additional supplemental data Immunity paper: NLRP3-mediated glutaminolysis controls microglial phagocytosis to promote Alzheimer’s disease progression

Secondary only controls for western blot(A) Microglia were primed with 100ng/ml LPS for 3 h, incubated with 1μM CRID3 or 100nM NP3-361 for 30 min and 5μM Aβ for 24 h. The lysates or (B) supernatants were prepared for immunoblot, and incubated with secondary only antibodies: goat anti-mouse 800 (used for NLRP3), goat anti-rabbit 680 (used for IL-1β) and goat anti-rat 680 (used for caspase-1). (C) The cortex of 4-, 6- and 12-month-old wild-type, APP/PS1, NLRP3-/- and APP/PS1.NLRP3-/- mice was prepared for immunoblot, and probed with secondary antibodies only. (D) Immunoblot analysis of secondary only controls from the cortex of patients with AD and non-demented, age-matched controls.