A high-quality chromosome-level genome assembly of Pacific white shrimp (Penaeus vannamei)

we sequenced the genome of the Pacific white shrimp using PacBio and Hi-C technologies, resulting in a 1.7Gb genome assembly with a Contig N50 of 57.65Kb and a Scaffold N50 of 605Kb. BUSCO analysis revealed that the genome assembly exhibited a gene coverage of 84.6%, which is considered high among crustacean reference genomes. In total, 907,237,377 bp of repetitive sequences (accounting for 50.48% of the assembled genome) and 22,923 protein-coding genes were identified. Utilizing Hi-C technology, we anchored 14,604 contigs onto 44 chromosomes, yielding a chromosome-level genome assembly with a contig N50 of 42,894,961 bp. In summary, our high-quality genome assembly provides valuable reference genomic resources for the improvement and breeding of elite cultivars of the Pacific white shrimp.