Human and mouse bone marrow CD45+ Erythroid cells have a constitutive expression of antibacterial immune response signature genes
Perik-Zavodskii, Roman;Perik-Zavodskaia, Olga;Nazarov, Kirill;Shevchenko, Julia;Alrhmoun, Saleh
Description
Single-cell protein expression of Arginase 1, CD36, CD45, CD49d, CD71, CD235a, CXCR4 (CD184) and Galectin 3 in adult human bone marrow Erythroid cells using flow cytometry.Cell preparation: Ficoll density-gradient centrifugation. Flow Cytometer: Attune NxT.Antibody panel:BioLegend: 125420 APC anti-mouse/human Mac-2 (Galectin 3/LGALS3), 306522 Brilliant Violet 605™ anti-human CD184 (CXCR4), 304328 APC/Cyanine7 anti-human CD49d, #334114 PerCP/Cyanine5. 5 anti-human CD71, #349104 FITC anti-human CD235a (Glycophorin A).Sigma-Aldrich: MABF1611 VioletFluor™ 450 Anti-human CD45 antibody.
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Metrics Over Time
Publication Details
Subfield
Physiology
Field
Medicine
Domain
Health Sciences
Confidence Score
53%
Source
Scholar Data Model
Keywords
Bone MarrowErythroid CellsErythroid Cells/immunologySingle-Cell Analysis