Microbiome profiling of bacterioplankton communities on sponge excurrent water, coral exudate water, and surface reef water.

Sponge exhalent water was collected from Looe Key reef, coral exudates water was collected from a coral incubation at Mote Marine lab, and ambient reef water was collected from Looe Key Reef surface water. All seawater was 0.2 um filtered to use as media in 2L bottles for an incubation experiment. Inoculum from reef surface water (1.6 um filtered) was used for the incubation. Bottles were incubated in the dark for 48 hours and samples were taken at the start (T0) and end (T48) for nutrient and microbiome analysis.