Additional file 12: of Microbiome and ecotypic adaption of Holcus lanatus (L.) to extremes of its soil pH range, investigated through transcriptome sequencing

Microbial-assigned transcripts, full results table, annotation and DESeq2 analysis. Description of File: The table includes information on expressed microbial transcripts. 1) Phylogenetic annotation of each transcript; 2) Whether significantly expressed in root acid bog soil (RA), root limestone soil (RL), shoot acid bog soil (SA) and/or shoot limestone soil (SL). Significantly expressed transcripts is defined as the number of microbial annotated transcripts that obtained ≥5 aligned reads in at least 3 samples in each of the treatments RA (total 8 samples), RL (total 9 samples), SA (total 10 samples), SL (total 9 samples); 3) two columns indicating whether the transcript is “upregulated in acid or limestone ecotype in acid bog soil” and/or “upregulated in acid or limestone ecotype in limestone soil” 4) KOG functional annotation (KOG ID, KOG definition, KOG class, KOG group) and best BLAST match (sequence length, database that contained the best match, accession number, gene description, best match organism, e-value, percentage ID, BLAST score, percentage sequence coverage); 5) The full DESeq2 result for each pairwise comparison in shoot (list 1-4) and root (list 5-8): these columns contain the log2foldchange and adjusted P value (FDR) for pairwise comparisons. Pairwise comparisons are coded as follows: soil (A = acid bog soil, L = limestone quarry soil), plant ecotype (a = acid bog plant ecotype, l = limestone quarry plant ecotype), shoots and roots (Shoot, Root), see sheet “DESeq2 pairwise comparisons” for further explanation of the 8 pairwise comparisons; 6) The count table. (XLSX 6498 kb)