P7 Kdr-KO scRNA seq data

Knutsen, Carsten

Description

Lung tissue from four experimental groups Kdrflox/floxand Tbx2icreKdrflox/floxunder maintained in normoxia or exposed to chronic hyperoxia were euthanized at P7. Lung tissue was dissected and snap-frozen in liquid nitrogen. Nuclei were prepared from frozen tissue using a nuclear isolation kit from 10X Genomics (10x Genomics, PN-1000494). For 10X Chromium, 10,000 nuclei were loaded per lane. The libraries were prepared according to manufacturer protocol (10X Genomics) using 3’V3 Kits (10X Genomics, PN-1000269) and were submitted for sequencing via a NovaSeq 6000 flow cell to a depth of 50,000 reads/cell. Raw sequencing data were processed using CellRanger pipeline

Citations (0)

Mentions (0)

Metrics

Dataset Index

0.5

FAIR Score

85%

Citations

0

Mentions

0

Metrics Over Time

Publication Details

DOI

Publisher

figshare

License

Creative Commons Attribution 4.0 International

Assigned Domain

Subfield

Molecular Biology

Field

Biochemistry, Genetics and Molecular Biology

Domain

Life Sciences

Confidence Score

40%

Source

Scholar Data Model

Keywords

Cell development, proliferation and death

Normalization Factors

FT

53.85

CTw

1.00

MTw

1.00