Description
Lung tissue from four experimental groups Kdrflox/floxand Tbx2icreKdrflox/floxunder maintained in normoxia or exposed to chronic hyperoxia were euthanized at P7. Lung tissue was dissected and snap-frozen in liquid nitrogen. Nuclei were prepared from frozen tissue using a nuclear isolation kit from 10X Genomics (10x Genomics, PN-1000494). For 10X Chromium, 10,000 nuclei were loaded per lane. The libraries were prepared according to manufacturer protocol (10X Genomics) using 3’V3 Kits (10X Genomics, PN-1000269) and were submitted for sequencing via a NovaSeq 6000 flow cell to a depth of 50,000 reads/cell. Raw sequencing data were processed using CellRanger pipeline
Citations (0)
No citations found
Mentions (0)
No mentions found
Metrics Over Time
Publication Details
DOI
Publisher
figshare
Subfield
Molecular Biology
Field
Biochemistry, Genetics and Molecular Biology
Domain
Life Sciences
Confidence Score
40%
Source
Scholar Data Model