Supplementary Table S1 for: Therapeutic potential of human adipose-derived stem cell exosomes in stress urinary incontinence – an in vitro and in vivo study

Results of GO analysisBackground/Aims:Toevaluate whether local injection of exosomes derived from human adipose-derivedstem cells (hADSCs) facilitates recovery of stress urinary incontinence (SUI)in a rat model.Methods: For the in vitrostudy, a Cell Counting Kit-8 (CCK-8) array and proteomic analysis wereperformed. For the in vivo study, female rats were divided into four groups:sham, SUI, adipose-derived stem cell (ADSC), and exosomes (n = 12 each). The SUI model was generated by pudendal nervetransection and vaginal dilation. Vehicle, hADSCs, or exosomes were injectedinto the peripheral urethra. After 2, 4, and 8 weeks, the rats underwent cystometrographyand leak point pressure (LPP) testing, and tissues were harvested forhistochemical analyses.Results: The CCK-8experiment demonstrated that ADSC-derived exosomes could enhance the growth ofskeletal muscle and Schwann cell lines in a dose-dependent manner. Proteomicanalysis revealed that ADSC-derived exosomes contained various proteins ofdifferent signaling pathways. Some of these proteins are associated with thePI3K-Akt, Jak-STAT, and Wnt pathways, which are related to skeletal muscle andnerve regeneration and proliferation. In vivo experiments illustrated that ratsof the exosome group had higher bladder capacity and LPP, and had more striatedmuscle fibers and peripheral nerve fibers in the urethra than rats of the SUIgroup. Both urethral function and histology of rats in the exosome group wereslightly better than those in the ADSC group. Conclusions: Local injection ofhADSC-derived exosomes improved functional and histological recovery after SUI.