Purification Of Recombinant Cas9
View DatasetDilworth, David;Harding, Rachel;Ravichandran, Mani;Barsyte-Lovejoy, Dalia
Description
SGC Open Notebook Project to Characterize the HMTase NSD3Exp027 Objective: To purify recombinant Cas9 protein for genome editing experiments. Recombinant Cas9 will be used with synthesized crRNA:tracrRNA to assemble ribonucleoproteins (RNPs) to target the nuclease activity of Cas9 to specific loci within the genome. Using RNPs as opposed to viral delivery of CRISPR gene editing components is advantageous as the expression of Cas9 is transient limiting off-target effects.
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Metrics Over Time
Publication Details
Subfield
Immunology
Field
Immunology and Microbiology
Domain
Life Sciences
Confidence Score
94%
Source
Open Alex