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Supplementary Figure 2A.Most abundant taxa of the oral microbiome in no caries and caries groups in adolescents at different taxonomic levels. (A-F) Barplot of the mean of the top 10 most abundant taxa by group: (A) at phylum; (B) at class; (C) at order; (D) at family; (E) at genus; (F) at species level. Significantly differentially abundant taxa are marked with (*) for MaAsLin, p ≤ 0.05 & LEfSe, LDA score ≥ 2.Supplementary Figure 2B. Analysis of regional variation and taxonomic markers of the oral microbiome in adolescents in no caries and caries groups. (A) Redundancy analysis (RDA) ordination showing the extent to which known variables account for taxonomic variation. ASV level, scaling=2. Ordination and biplot are standard scaled. (B) Overlap between regional and overall taxonomic markers. MaAsLin, p ≤ 0.05 & LEfSe, LDA score ≥ 2. (C-F) Effect size barplot of taxonomic markers in different regions at the species level. LEfSe LDA: (C) in Astana region (Ast); (D) in Kokshetau region (Kok); (E) in Semey region (Sem); (F) in Oskemen region (Osk).