Effect of Different Culture Media on Growth of Microalgae Haematococcus sp. TISTR 9450RE

This study aimed to select the appropriate medium for supporting the best growth of Haematococcus sp.TISTR 9450RE They were BG-11, Bold's Basal, CA and N8.The algae were cultured in 500 ml Erlenmeyer flask containing 200 ml of each medium. The initial cell concentration was 0.1 OD at 680 nm (equivalent to 0.07 g/L of cell dry weight). All flasks were shaken continuously at 100 rpm. At 7 and 14 days of cultivation, the biomass content of 0.1 and 0.13 g/L were obtained from BG-11 and Bold's Basal medium, respectively. Whereas slightly lower cell concentration at day 7 were 0.09 and 0.1 g/L from CA and N8 medium, and at day 14 were 0.11 and 0.12 g/L, respectively. The highest biomass content was found in BG-11 0.32 g/L after 28 days cultivation, with 4.57 times increasing from day 0, followed by Bold's Basal, N8 and CA at 0.26, 0.24 and 0.22 g/L, with 3.43, 3.25 and 3.14 times, respectively. Due to cell division, the highest absorbance of 0.44 at day 28 was found in N8, but biomass was less. The pH value of BG-11, Bold's Basal, CA and N8 at the end of cultivation were 11.06, 10.92, 10.74 and 7.49, respectively. The results revealed that BG-11 was the suitable medium for culturing Haematococcus sp. TISTR 9450RE and used for further study of carotenoid production from Haematococcus sp. TISTR 9450RE.