Transcriptome Analysis of Silver Carp (Hypophthalmichthys molitrix) by Paired-End RNA Sequencing

In this study, we performed de novo transcriptome assembly and analysis of the 17.10 million short-read sequences produced by Illumina paired-end sequencing technology. Using an improved multiple k-mer contig assembling methods coupled with further scaffolding, 85759 sequences were obtained. There were 23044 sequences annotated with 3423 GO terms for 104196 term occurrences and the three corresponding organizing principles. A total of 38200 assembled sequences were involved in 218 predicted KEGG metabolic pathways. We also recovered 41 genes out of 44 genes involving in the biosynthesizing of glutathione and out of which 8 genes were identified having experienced positive selection between silver carp and zebrafish using likelihood ratio test.