Supplementary Data for "Arabidopsis DXO1 is an evolutionarily diverged homolog that impacts ribosome loading and mRNA surveillance"

Supplementary tables and files used for Arabidopsis pre-rRNA processing analysis.File Descriptions:Supplemental tables: Supplementary tables containing evolutionary analysis dataset, descriptions of sequencing samples, gene lists, and primer details. 45S_rDNA_Arabidopsis_Sims2021.fa: 45S rDNA reference based on sequencing NOR2 (1)45S_rDNA_Arabidopsis_Sims2021.fa.fai: index file generated for the 45S rDNA sequence45S_rDNA_Arabidopsis_Sims2021.fa.len: file containing the length of the 45S rDNA sequence45S_rDNA_Arabidopsis.gff: annotation of 45S rDNA features in GFF formatThe original 45S rDNA reference sequence was generated previously (1). The GFF annotation was based on homology to characterized rRNA sequences (2,3) and pre-defined cleavage sites (4) with some adjustments made based on observed 5′-P ends near the following endonucleolytic cleavage sites: P, P′, P₂, and A₃.ReferencesJ. Sims, G. Sestini, C. Elgert, A. von Haeseler, P. Schlögelhofer, Sequencing of the Arabidopsis NOR2 reveals its distinct organization and tissue-specific rRNA ribosomal variants. Nat Commun 12, 387 (2021).I. Unfried, P. Gruendler, Nucleotide sequence of the 5.8S and 25S rRNA genes and of the internal transcribed spacers from Arabidopsis thaliana. Nucleic Acids Res 18, 4011 (1990).I. Unfried, U. Stocker, P. Gruendler, Nucleotide sequence of the 18S rRNA gene from Arabidopsis thaliana Co10. Nucleic Acids Res 17, 7513 (1989).J. Sáez-Vásquez, M. Delseny, Ribosome Biogenesis in Plants: From Functional 45S Ribosomal DNA Organization to Ribosome Assembly Factors. Plant Cell 31, 1945–1967 (2019).