Version v1.2.1-10k

Supplementary Data - Nanopore cDNA With and Without mtDNA

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Eccles, David Andrew;Grasso, Carole;Lamiable, Olivier;McConnell, Melanie;Berridge, Mike

Description

Update: added 10k of FASTQ reads from a more recent run (part of the same differential expression project).Supplementary data to go with David Eccles' poster about nanopore cDNA sequencing with and without mitochondrial DNA.Reference mouse mtDNA and mtDNA reads from 4T1 cells (from MinION cDNA sequencing, and from SRA run SRR6747859).fwd_4T1_BC06.correctedReads.uniqueOnly.fasta.gz -- Canu-corrected cDNA reads from Wildtype 4T1 strain, filtered as transcript-forward orientationrev_4T1_BC06.correctedReads.uniqueOnly.fasta.gz -- Canu-corrected cDNA reads from Wildtype 4T1 strain, filtered as transcript-reverse orientationfwd_4T1_BC07.correctedReads.uniqueOnly.fasta.gz -- Canu-corrected cDNA reads from 4T1ρ0 strain (no mitochondrial DNA), filtered as transcript-forward orientationrev_4T1_BC07.correctedReads.uniqueOnly.fasta.gz -- Canu-corrected cDNA reads from 4T1ρ0 strain (no mitochondrial DNA), filtered as transcript-reverse orientationOther intermediate data files are also included, see 4way_mapping_process.txt for more details.The mpileup differential coverage script can be found here.

Citations (0)

Mentions (0)

Metrics

Dataset Index

0.3

FAIR Score

79%

Citations

0

Mentions

0

Metrics Over Time

Publication Details

DOI

Publisher

Zenodo

Assigned Domain

Subfield

Biomedical Engineering

Field

Engineering

Domain

Physical Sciences

Confidence Score

99%

Source

Open Alex

Keywords

nanoporeilluminacDNAstranded

Normalization Factors

FT

13.46

CTw

1.00

MTw

1.00