Data from: Multiplexed microsatellite markers for genetic studies of beech
View DatasetDescription
European beech (Fagus sylvatica L.) is one of the economically most important broadleaved tree species in Europe and has become a model for studying climate change effects. Multiplex PCR of microsatellites is a fast and cost-effective technique allowing high throughput genotyping. Here we present the procedure used to develop two multiplex kits (8-plexes) for European beech. We paid particular attention to quality control throughout all steps of the multiplex kits development (null allele detection, error rate measurements, linkage disequilibrium). Preliminary assays suggest that the multiplex kits amplify 16 loci largely devoid of null alleles and allow rapid and cost-effective genotyping of beech with low error rates. The two kits, which differ in their levels of polymorphism, most likely due to marker origin, were also informative in seven other beech species tested.
Citations (1)
- https://doi.org/10.1111/j.1755-0998.2011.03094.xDataCite MDC
Cited on 07 December 2011
Weight: 1.00
Mentions (0)
No mentions found
Metrics Over Time
Publication Details
Subfield
Clinical Biochemistry
Field
Biochemistry, Genetics and Molecular Biology
Domain
Life Sciences
Confidence Score
40%
Source
Scholar Data Model