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Data from: Multiplexed microsatellite markers for genetic studies of beech

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Lefèvre, Sophie;Wagner, Stefanie;Petit, Rémy J.;de Lafontaine, Guillaume

Description

European beech (Fagus sylvatica L.) is one of the economically most important broadleaved tree species in Europe and has become a model for studying climate change effects. Multiplex PCR of microsatellites is a fast and cost-effective technique allowing high throughput genotyping. Here we present the procedure used to develop two multiplex kits (8-plexes) for European beech. We paid particular attention to quality control throughout all steps of the multiplex kits development (null allele detection, error rate measurements, linkage disequilibrium). Preliminary assays suggest that the multiplex kits amplify 16 loci largely devoid of null alleles and allow rapid and cost-effective genotyping of beech with low error rates. The two kits, which differ in their levels of polymorphism, most likely due to marker origin, were also informative in seven other beech species tested.

Citations (1)

Mentions (0)

Metrics

Dataset Index

2.2

FAIR Score

77%

Citations

1

Mentions

0

Metrics Over Time

Publication Details

DOI

Publisher

Dryad

Assigned Domain

Subfield

Clinical Biochemistry

Field

Biochemistry, Genetics and Molecular Biology

Domain

Life Sciences

Confidence Score

40%

Source

Scholar Data Model

Keywords

multiplex PCRRecentquality controlsFagus sylvatica

Normalization Factors

FT

13.46

CTw

1.00

MTw

1.00