Raw K562 experimental data and miRNA–Hippo interaction table for the imatinib–Hippo–miRNA CML study

Raw experimental data and miRNA–Hippo interaction table for “Time-resolved Hippo–YAP and microRNA responses to imatinib in CML K562 cells with in silico validation in CD34⁺ CML progenitors” Description of the data and file structure# Data for imatinib–Hippo–miRNA study in K562 cellsRaw experimental data and interaction tables underlying the manuscript:Akbari S, et al. “Time-resolved Hippo–YAP and microRNA responses to imatinib in CML K562 cells with in silico validation in CD34⁺ CML progenitors” (PLOS ONE, in submission).## Files- K562_MTT_viability_raw.xlsxRaw absorbance values for 48 h MTT viability assays at multiple imatinib concentrations.- K562_AOEB_apoptosis_counts_raw.xlsxRaw AO/EB cell counts for apoptosis quantification at 48 h (control vs imatinib).- K562_ROS_DCFHDA_raw.xlsxRaw DCFH-DA fluorescence readings for ROS measurements over time.- K562_gene_qPCR_Ct_raw.xlsxRaw Ct values for Hippo pathway genes and GAPDH reference.- K562_miRNA_qPCR_Ct_raw.xlsxRaw Ct values for Hippo-related microRNAs and U6 reference.- miRNA_targets_master.csvCurated miRNA–target interaction table used for the Hippo/miRNA network.- PhaseContrast_200x_control_48h_raw.tif- PhaseContrast_200x_imatinib_48h_raw.tif- AOEB_control_raw_48h.tif- AOEB_imatinib_raw_48h.tifRaw phase-contrast and AO/EB microscopy images corresponding to morphology and apoptosis panels.## CodeAll R scripts for processing GSE12211/GSE14671 and reproducing the bioinformatics analyses are available at:https://github.com/soroushakbari/cml-hippo-imatinib-k562