TRIMELvax – Data Repository

Raw experimental data accompanying the manuscript: Pérez-Baños et al. "Antigen-Presenting Cell-like Neutrophils Orchestrate TRIMELVax-Induced Antitumor Immunity by Linking Early Inflammation to Tumor Growth Control".Analysis codeThe R scripts for the RT-qPCR and flow cytometry statistical analyses are available on GitHub:🔗 https://github.com/astaburuaga-garcia/TRIMELVax FilesRT-qPCR dataFileDescriptionRTqPCR_CTvalues.csvRaw Ct values from the Mouse Inflammatory Cytokines & Receptors RT² Profiler PCR Array (Qiagen, cat. PAMM-011Z). One column per sample, rows are array wells. Semicolon-delimited.Column naming convention: (e.g., TrimelVax_N1_6).Housekeeping wells: H01–H05 (used for ΔCt normalisation). Remaining row-H wells are array controls.Batches: Replicates N1/N2 were processed in batch 1; N3/N4 in batch 2. B16Vax samples were run in batch 2 only.Flow cytometry dataAll four files share the same structure: columns for cell_type, time.point, treatment, and Value (cell count). Semicolon-delimited.FileDescriptionflow1_APC_totales.csvTotal antigen-presenting cell (APC) counts.flow2_poblaciones_CDs.csvDendritic cell (DC) subpopulation counts.flow3_sub_CDs_CFSE_pos.csvCFSE⁺ (vaccine-derived) DC subpopulation counts.flow4_sub_CDs_CFSE_neg.csvCFSE⁻ (host-derived) DC subpopulation counts.Treatments: PBS, Gvax, TRIMELVax, TRIMELVax +Ly6G.Timepoints: 12, 24, 48, 72 h post-vaccination.Decimal separator: Comma (European convention). The analysis scripts handle the conversion automatically.GraphPad Prism source dataSource data for individual figure panels, provided as GraphPad Prism files (.pzfx).FileDescriptionData Figure 2 Panel B.pzfxSource data for Figure 2B.Data Figure 2 Panel F.pzfxSource data for Figure 2F.Data Figure 3 Panel F.pzfxSource data for Figure 3F.Data Figure 4 Panel B,D and F.pzfxSource data for Figure 4B, 4D, and 4F.Data Figure 5 Panel B.pzfxSource data for Figure 5B.Data Figure 5 Panel C.pzfxSource data for Figure 5C.Data Supplementary Figure 2 Panel B.pzfxSource data for Supplementary Figure 2B.Data Supplementary Figure 3 Panel B.pzfxSource data for Supplementary Figure 3B.Data Supplementary Figure 3 Panel D.pzfxSource data for Supplementary Figure 3D.Data Supplementary 4.pzfxSource data for Supplementary Figure 4.