Cellular responses to sulfated galactans from Gracilaria corticata: cytotoxicity and immune function in HDF, HaCaT, RAW264.7, and B16-F10 cells

Dataset 1: Cytotoxicity, metabolic activity, and SOD activity of sulfated galactans from Gracilaria corticata in human dermal fibroblasts (HDF)Description: This dataset includes raw data from three experiments assessing the effects of six sulfated galactan fractions (GC-1A, GC-1B, GC-2A, GC-3A) on HDF cells. MTT assay evaluated cell viability across concentrations (1.00–0.02 µg/µL) at 24 h. alamarBlue assay measured metabolic activity (cell proliferation) at 0.5 µg/µL over 24, 48, and 72 h. SOD activity was quantified at 0.5 µg/µL after 24 h. All data are normalized to non-treated controls (100%). Statistical analyses used one-way ANOVA with Dunnett's or Šídák's post-hoc tests (mean ± SEM, n=4).Dataset 2: Cytotoxicity and metabolic activity of sulfated galactans from Gracilaria corticata in human keratinocytes (HaCaT)Description: This dataset contains raw data from two experiments evaluating GC fractions (GC-1A, GC-1B, GC-2A, GC-3A) on HaCaT keratinocytes. MTT assay assessed cytotoxicity across concentrations (0.50–0.01 µg/µL) at 24 h. alamarBlue assay measured metabolic activity (cell proliferation) at 0.5 µg/µL over 24, 48, and 72 h. Values are normalized to non-treated controls (100%). Statistical analyses used one-way ANOVA with Dunnett's or Šídák's post-hoc tests (mean ± SEM, n=4).Dataset 3: Cytotoxicity, metabolic activity, phagocytosis, and nitric oxide production of sulfated galactans from Gracilaria corticata in RAW264.7 macrophagesDescription: This dataset includes raw data from four experiments testing GC fractions (GC-1A, GC-1B, GC-2A, GC-3A) on RAW264.7 macrophages. MTT assay measured viability across concentrations (0.50–0.01 µg/µL) at 24 h. alamarBlue assessed metabolic activity (cell proliferation) at 0.5 µg/µL over 24, 48, and 72 h. Phagocytosis (neutral red uptake) and NO production (Griess reagent) were measured at 0.5 µg/µL after 24 h. Data are normalized to non-treated controls (100%). Statistics used one-way ANOVA with Dunnett's or Šídák's post-hoc tests (mean ± SEM, n=4).Dataset 4: Cytotoxicity and metabolic activity of sulfated galactans from Gracilaria corticata in B16-F10 melanoma cellsDescription: This dataset contains raw data from two experiments evaluating GC fractions (GC-1A, GC-1B, GC-2A, GC-3A) on B16-F10 melanoma cells. MTT assay assessed anti-melanoma activity across a broad concentration range (0.50–0.0002 µg/µL) at 24 h. alamarBlue assay measured metabolic activity (cell proliferation) at 0.5 µg/µL over 24, 48, and 72 h. Values are normalized to non-treated controls (100%). Statistical analyses used one-way ANOVA with Dunnett's or Šídák's post-hoc tests (mean ± SEM, n=4).