Comparison of isolation methods using commercially available kits for obtaining extracellular vesicles from cow milk (supplementary files)

Supplementary Figure 1. Measurement of EV counts and protein concentrations in each fraction from qEV. (A) Combination of centrifugation-based casein removal and qEV. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). (B) Combination acetic acid precipitation-mediated casein removal and qEV. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). (C) Combination of EDTA precipitation-mediated casein removal and qEV. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). Supplementary Figure 2. Measurement of EV counts and protein concentration in each fraction from EVSecond L70. (A) Combination of centrifugation-mediated casein removal and EVSecond L70. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3); (B) Combination of acetic acid precipitation-mediated casein removal and an EVSecond L70. Black circles represent EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3). (C) Combination of EDTA precipitation-mediated casein removal and EVSecond L70. Black circles EV counts/mL; squares represent the protein concentration at Abs = 280 nm. Values are means ± SEM (n = 3).