CIL:37106, Xenopus laevis, CNS neuron (sensu Vertebrata). In Cell Image Library

This is IMAGE #4 (day 3) of a developing neuron from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47. The cell was imaged daily over 3 days and shows the development of the dendritic arbor and axon over time. This is a lower zoom image of same time point shown in IMAGE #3. The cell was transfected with in vivo electroporation of plasmid DNA encoding eGFP ~24 hours before the image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.