Green turtles are long-lived and may take up to 59 years to reach sexual maturity. Undertaking tremendous feats of navigation, adults return to the same beach to breed each season.
DNA from the green sea turtle was collected in Hong Kong. We sequenced the 2.24 Gb genome to a depth of approximately 82.3 X with short reads from a series of libraries with various insert sizes ( 170bp, 500bp, 800bp, 2kb, 5kb, 10kb,20kb and 40kb) on a HiSeq 2000 sequencer.
The assembled scaffolds of high quality sequences total 180.94 Gb, with the contig and scaffold N50 values of 20.4 kb and 3.78 Mb respectively. We identified 19,633 protein-coding genes with an mean length of 1456 bp.Experimental procedures and animal care were conducted in strict accordance with guidelines approved by the RIKEN Animal Experiments Committee (Approval IDs H14-23 and H16-10).

The soft shell turtle can reach a carapace length of 1 ft (0.30 m). It has webbed feet for swimming. They are called "softshell" because their carapace lacks horny scutes (scales). The carapace is leathery and pliable, particularly at the sides. It is commercially farmed in vast numbers for the food trade.
DNA from the soft shell turtle was collected in Japan. We sequenced the 2.21 Gb genome to a depth of approximately 105.6 X with short reads from a series of libraries with various insert sizes ( 170bp, 500bp, 800bp, 2kb, 5kb, 10kb,20kb and 40kb) on a HiSeq 2000 sequencer.
The assembled scaffolds of high quality sequences total 221.7 Gb, with the contig and scaffold N50 values of 21.9 kb and 3.33 Mb respectively. We identified 19,327 protein-coding genes with an mean length of ~1500bp. Experimental procedures and animal care were conducted in strict accordance with guidelines approved by the RIKEN Animal Experiments Committee (Approval IDs H14-23 and H16-10).

The black flying fox, Pteropus alecto, is a megabat in the family Pteropodidae. Members of the genus Pteropus include the largest bats in the world. The Pteropus genus currently has about 57 recognised species. The genus is primarily an island taxon, with 55 species having some or all of their distribution on islands.
We applied a whole genome shotgun strategy and next-generation sequencing technologies using an Illumina HiSeq 2000 platform to sequence the genome of an individual adult male P. alecto. In order to lower the risk of non-randomness, we constructed 26 paired-end libraries sequenced on 26 lanes, with insert sizes of about 250 base pairs (bp), 500 bp, 800 bp, 2 kb, 5 kb, 10 kb and 20 kb. In total, we generated about 219.7 Gb (109.8x coverage) of high quality sequence for the assembly. The genome was de novo assembled by SOAPdenovo. The final total contig size and N50 were 1.99 Gb and 25.7 kb, respectively. The total scaffold size and N50 were 2.03 Gb and 15.8 Mb, respectively.

Brandt's bat (Myotis brandtii) is a species of vesper bat in the family Vespertilionidae. It is found throughout most of Europe and parts of Asia. It is known for its extreme longevity quotient, approximately twice that of humans.
A whole-genome shotgun strategy was applied to sequence the genome of an adult male Brandts bat (M. brandtii) from the Obvalnaya cave in Russia. We also sequenced liver, kidney and brain transcriptomes of hibernating and summer-active M. brandtii. Approximately 156 Gb (or 78 × ) genome data including 101 Gb (or 51 × ) short insert size reads were generated, resulting in a high-quality assembly with scaffold N50 of 3.1 Mb and contig N50 of 21 kb

Domestic yaks (Bos grunniens) provide meat and other necessities for Tibetans living at high altitude on the Qinghai-Tibetan Plateau and in adjacent regions. Here, we present the draft genome sequence of a female domestic yak generated using Illumina HiSeq 2000 technology at 65-fold coverage.
De novo assembly of 4.4 billion reads from paired-end libraries yielded a draft assembly with a total length of 2,657 Mb, and contig and scaffold N50 sizes of 20.4 kb and 1.4 Mb, respectively. Approximately 90% of the total sequence was covered by 2,083 scaffolds of >307 kb, with the largest scaffold spanning 8.8 Mb.

The microbats constitute the suborder Microchiroptera within the order Chiroptera (bats). They are most often referred to by their scientific name. Other English names are "insectivorous bats", "echolocating bats", "small bats" or "true bats". All these names are somewhat inaccurate, because not all microbats feed on insects, and some of them are larger than small megabats.
We applied a whole genome shotgun strategy and next-generation sequencing technologies using an Illumina HiSeq 2000 platform to sequence the genome of an individual adult male M. davidii. In order to lower the risk of non-randomness, we constructed 26 paired-end libraries sequenced on 26 lanes, with insert sizes of about 250 base pairs (bp), 500 bp, 800 bp, 2 kb, 5 kb, 10 kb and 20 kb. In total, we generated about 237.2 Gb (118.6x coverage) of high quality sequence for the assembly. The genome was de novo assembled by SOAPdenovo. The final contig size and N50 were 1.91 Gb and 13.2 kb, respectively. The total scaffold size and N50 were 2.09 Gb and 3.39 Mb, respectively.

The Pacific oyster (Crassostrea gigas) belongs to one of the most species-rich but genomically poorly explored phyla, the Mollusca. In this study, DNA was extracted from an oyster derived from four generations of full-sib mating. Short-reads and a fosmid-pooling strategy were used for sequencing and assembly of the oyster genome. DNA extracted from the inbred oyster and another wild oyster was used for resequencing analysis. Finally, RNA-seq and small RNA from different organs at different developmental stages were extracted for developmental studies.

The pig (Sus scrofa) is an economically important food source and also serves as an important model organism. Here we present the sequence of one male inbred Wuzhishan pig (WZSP) at generation F20. DNA was extracted from the ear of this species. We applied a whole genome shotgun strategy and next-generation sequencing technologies using Illumina HiSeq 2000 for sequencing, in total 340.53 Gb of raw data were generated and 210.79 Gb high quality data were used for assembly. The assembly result of WZSP was 2.6 Gbp, and the N50 size of contigs and scaffolds were 23.5 Kbp and 5.4 Mbp, respectively. 38% of the genome was identified as repeats and 20,326 genes were identified from homolog prediction.

The Chinese rhesus macaque (Macaca mulatta lasiota) is a subspecies of rhesus macaques that mainly resides in western and central China. Due to their anatomical and physiological similarity with human beings, macaques are a common laboratory model. Also, as several macaques species have been sequenced, such as the Indian rhesus macaque and the crab-eating macaque, examination of the Chinese rhesus macaque (CR) genome offers interesting insights into the entire Macaca genus.The DNA sample for data sequencing and analyses was obtained from a five-year old female CR from southwestern China. The genome was sequenced on the IlluminaGAIIx platform, from which 142-Gb of high-quality sequence, representing 47-fold genome coverage for CR. The total size of the assembled CR genome was about 2.84 Gb, providing 47-fold on average. Scaffolds were assigned to the chromosomes according to the synteny displayed with the Indian rhesus macaque and human genome sequences. About 97% of the CR scaffolds could be placed onto chromosomes.

Here is the genome of the naked mole rat (Heterocephalus glaber), one of the only two known eusocial mammals. It is a fascinating species, due not only to its unique behavior but also to its unique physiology. Among its unusual characteristics, it has the longest lifespan of all rodent species, is a poikilotherm, is highly resistant to cancer, and does not sense certain types of pain.The genome of an individual male naked mole rat was sequenced on the Illumina HiSeq 2000 platform and assembled using SOAPdenovo. In assembly 2.5Gb (gigabase pairs) contig sequences with N50 19.3kb (kilobase pairs) and N90 4.7kb, and 2.7Gb scaffold sequences with N50 1.6Mb (megabase pairs) and N90 0.3Mb was obtained.